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Urease Positive Bacteria

[Summary]Welcome to Microbugz Urease Test Urease broth is a differential medium that tests the ability of an organism to produce an exoenzyme, called urease, that hydrolyzes urea to ammonia and carbon dioxide. The broth contains two pH buffers, urea, a very s

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Urease Test

Urease broth is a differential medium that tests the ability of an organism to produce an exoenzyme, called urease, that hydrolyzes urea to ammonia and carbon dioxide. The broth contains two pH buffers, urea, a very small amount of nutrients for the bacteria, and the pH indicator phenol red. Phenol red turns yellow in an acidic environment and fuchsia in an alkaline environment. If the urea in the broth is degraded and ammonia is produced, an alkaline environment is created, and the media turns pink.

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The Virtual Edge

STAPHYLOCOCCUS :

1. Gram-positive cocci, arranged in grape-like clusters

2. Catalase positive

3. High salt tolerance

4. Facultative anaerobes

Three common species are...

1. Staphylococcus aureus

2. Staphylococcus epidermidis

3. Staphylococcus saprophyticus

Staphylococcus spp. are normal flora of skin and mucous membranes, however they can cause disease when introduced into normally sterile sites in the body. Staphylococcus aureus is the most pathogenic species in the group. The virulence of Staphylococcus aureus is due to a number of enzymes or toxins that some strains can produce.

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